Improving the security of critical road infrastructure is a major task for owners and operators of tunnels and bridges in the European TEN-T Network (Trans-European Networks of Transport) (European Parliament and Council 1996). Up to now, there has not been a systematic procedure for identifying and assessing critical infrastructure objects and selecting appropriate protection measures. The EC FP7 project SeRoN for the first time presents an innovative methodology in order to support road owners and operators in handling this complex task. This paper describes the methodology and project results in detail by giving an introduction into its practical application.